Background: Natural products made from plant sources have been used in a variety of cosmetic applications as a\nsource of nutrition and as a whitening agent. The flowers of Cassia fistula L, family Fabaceae, have been used as a\ntraditional medicine for skin diseases and wound healing and have been reported to possess anti-oxidant\nproperties. The anti-aging effect of C. fistula flower extract on human skin fibroblast was investigated.\nMethods: The butanolic extraction of C. fistula flowers was completed and the active compounds were classified.\nThe cytotoxicity of fibroblasts was evaluated by SRB assay for the purposes of selecting non-toxic doses for further\nexperiments. The collagen and hyaluronic acid (HA) synthesis was then measured using the collagen kit and ELISA,\nrespectively. Moreover, the enzyme activity, including collagenase, matrixmelloproteinase-2 (MMP-2) and tyrosinase,\nwere also evaluated.\nResults: It was found that the flower extract did not affect skin fibroblast cell growth (IC50 > 200 Ã?¼g/mL). The results\ndid show that the flower extract significantly increased collagen and HA synthesis in a dose dependent manner.\nThe flower extract (50ââ?¬â??200 Ã?¼g/mL) also significantly inhibited collagenase and MMP-2 activity. Furthermore, this\nflower extract could inhibit the tyrosinase activity that causes hyperpigmentation, which induces skin aging.\nConclusions: The C. fistula flower extract displayed a preventive effect when used for anti-aging purposes in\nhuman skin fibroblasts and may be an appropriate choice for cosmetic products that aim to provide whitening\neffects, and which are designated as anti-aging facial skin care products.
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